Coding

Part:BBa_K3945005:Design

Designed by: Ramin Kahidi   Group: iGEM21_Calgary   (2021-10-20)


Binding Affinity Optimized Mutated Lanmodulin I (BAO LanM-I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal BamHI site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Likely modifications that improve protein stability were identified using HotSpot Wizard. These mutations were conducted to generate a large mutant library. Structural models were obtained to work with further. The members of this dictionary then were protonated at several different pH levels. The binding potential was then calculated for each pH level. The proteins with improved binding throughout the pH range were taken from the crop, and the rest were discarded. The two sequences with the highest binding potential throughout the range were selected to be shared so other researchers could utilize their improved binding and stability for different processes.